Coenzyme Q10 (CoQ10) is a potent antioxidant against oxidative damage to skin. But the amount of CoQ10 in tissue is reduced with age. To supply the reduction, we evaluated some formulations of CoQ10-γCD Complex in vitro skin absorption experiment and found that both aqueous solubility and absorption of CoQ10 in the tissue culture were significantly enhanced by the combination of CoQ10-γCD Complex with dipotassium glycyrrhizate (GZK2).
Effect of CoQ10 on human skin
Some skin problems were improved by the oral intake of high absorption CoQ10-γCD Complex.
Is it possible to complement CoQ10 to skin directly?
The solubilization study of CoQ10 was made by adding various solubilizing agents as the third ingredient to CoQ10 or CoQ10-γCD Complex.
Furthermore, the effect of solubilization of CoQ10 was estimated in the skin absorption experiment.
Solubilization of CoQ10 by solubilizing agents
Various solubilizing agents ware added to CoQ10 or CoQ10-γCD complex aqueous suspension, and then the concentration of CoQ10 in water was measured by HPLC.
Solubilization of CoQ10 by GZK2
GZK2 was added to CoQ10 or CoQ10-γCD complex aqueous suspension, and then the concentration of CoQ10 in water was measured by HPLC.
"LabCyte EPI-MODEL" The human three-dimensional cultured epidermis model
The human three-dimensional cultured epidermis model
"LabCyte EPI-MODEL (Japan Tissue Engineering Co., Ltd.) "
≫ This is multi-layered epidermis model cultured by using normal human skin cells.
≫ It's characterized by the morphological resemblance to human skin.
≫ It consisted of stratum basale, stratum spinosum, stratum granulosum and stratum corneum.
≫ It's in no danger of species difference, since human cells are utilized.
≫ Test results are reproducible because of small well-to-well and lot-to-lot variation.
Procedure of skin absorption experiment
Uptake of CoQ10 to epidermis
The uptake of CoQ10 to human three-dimensional culture epidermis "LabCyte EPI-MODEL" in various test materials was measured by HPLC.
|≫||The solubility of CoQ10 was increased by the combination of CoQ10-γCD complex and GZK2, then it is confirmed that very high solubility more than 2,000 μg/mL can be reached. On the other hand, in case of not complexed CoQ10, solubility of CoQ10 was not increased in spite of GZK2 addition.|
|≫||It may be assumed that the intermolecular force between CoQ10 molecules can be reduced by the complexation with γCD.|
|≫||In the skin absorption experiment, the uptake of CoQ10 to epidermis was increased by the combination of CoQ10-γCD complex and GZK2.|