No. 16 New Synbiotics by α-cyclodextrin and probiotics
Synbiotics?
Synbiotics refer to nutritional supplements combining probiotics (probiotics are live microorganisms thought to be healthy for the host organism) and prebiotics (prebiotics are non-digestible food ingredients that stimulate the growth and / or activity of bacteria in the digestive system which are beneficial to the health of the body) in a form of synergism.
Probiotics are not stimulated by prebiotics, which would mean symbiosis.
The combination of the two may give a synergistic effect for health.
α-cyclodextrin?
α-cyclodextrin (α-CD) is classified as water-soluble dietary fiber, and it is functional foods material which have a weight reduction effect, a constipation improvement effect, and a decrease effect of cholesterol, triglyceride, hyperglycemia. It arrives at the large intestine, and used to probiotics selectively.
On the other hand, lactobacillus and bifidobacteria have been already well known as probiotics. Prebiotics are usually digested by probiotics.
If prebiotics has stability in the existence of probiotics and can become the bait of probiotics only in the body, it is enable use as synbiotics by the combination.
Bacterial Growth Medium
GYP medium (PH7.0)
- D-Glucose 2%
- Yeast Extract 0.5%
- Polypeptone 1%
YP medium (PH7.0)
- Yeast Extract 0.5%
- Polypeptone 1%
carbon source is only CD
Test strain from commercially available yogurts
- Lactobacillus casei strain shirota YIT 9029 from Yakult
- Lactobacillus casei from Meiji
- Lactobacillus Gasseri OLL2716 } from KAGOME
- Bifidobacterium bifidum
Lactobacillus casei strain shirota YIT 9029
Lactobacillus casei
Lactobacillus Gasseri OLL2716 / Bifidobacterium bifidum
Growth promoting effect by intake of α-CD
It was recognized that the Bifidobacterium increase more than 3 times in stool after intake of α-CD of 3g per day for 3 weeks. (n = 10)
Citation from Wacker Chemical Corporation
Frequent bowel movement by intake of α-CD
Frequency of defecation increased more than 1.5 times after intake of α-CD of 3g per day. (n = 10)
Citation from Wacker Chemical Corporation
Conclusion
- The growth of bacteria was the slowest by adding α-CD.
But Lactobacillus casei strain shirota can multiply with α-CD as carbon source without glucose.
→ α-CD was hard to be digested by probiotics, it means α-CD is more stability than other prebiotics. - It was recognized that the Bifidobacterium increase more than 3 times in stool and frequent bowel movement more than 1.5 times after intake of α-CD of 3g per day for 3 weeks. (Wacker Chemical Corporation)
→ α-CD is assimilated slowly, and finally become nutrient of probiotics coexistence with various organic compounds and bacteria in intestinal environment.
α-CD as prebiotics can be used to ingredient of synbiotics.