Recent research finding
No.16 New Synbiotics by α-cyclodextrin and probiotics

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Synbiotics?

Synbiotics refer to nutritional supplements combining probiotics (probiotics are live microorganisms thought to be healthy for the host organism) and prebiotics (prebiotics are non-digestible food ingredients that stimulate the growth and / or activity of bacteria in the digestive system which are beneficial to the health of the body) in a form of synergism.

Probiotics are not stimulated by prebiotics, which would mean symbiosis.
The combination of the two may give a synergistic effect for health.

α-cyclodextrin?

α-cyclodextrin (αCD) is classified as water-soluble dietary fiber, and it is functional foods material which have a weight reduction effect, a constipation improvement effect, and a decrease effect of cholesterol, triglyceride, hyperglycemia. It arrives at the large intestine, and used to probiotics selectively.

On the other hand, lactobacillus and bifidobacteria have been already well known as probiotics. Prebiotics are usually digested by probiotics.

If prebiotics has stability in the existence of probiotics and can become the bait of probiotics only in the body, it is enable use as synbiotics by the combination.

Bacterial Growth Medium

GYP medium (PH7.0)
・D-Glucose 2%
・Yeast Extract 0.5%
・Polypeptone 1%
YP medium (PH7.0)
・Yeast Extract 0.5%
・Polypeptone 1%
   ※carbon source is only CD

Test strain from commercially available yogurts

Lactobacillus casei strain shirota YIT 9029 from Yakult
Lactobacillus casei from Meiji
Lactobacillus Gasseri OLL2716 } from KAGOME
Bifidobacterium bifidum

Lactobacillus casei strain shirota YIT 9029

Carbon source : only CD

Carbon source : CD and glucose

Lactobacillus casei

Carbon source : only CD

Carbon source : CD and glucose

Lactobacillus Gasseri OLL2716 / Bifidobacterium bifidum

Carbon source : only CD

Carbon source : CD and glucose

Growth promoting effect by intake of αCD

It was recognized that the Bifidobacterium increase more than 3 times in stool after intake of αCD of 3g per day for 3 weeks. (n = 10)

Citation from Wacker Chemical Corporation

Frequent bowel movement by intake of αCD

Frequency of defecation increased more than 1.5 times after intake of αCD of 3g per day. (n = 10)

Citation from Wacker Chemical Corporation

Conclusion

The growth of bacteria was the slowest by adding αCD.
But Lactobacillus casei strain shirota can multiply with αCD as carbon source without glucose.
⇒ αCD was hard to be digested by probiotics, it means αCD is more stability than other prebiotics.
It was recognized that the Bifidobacterium increase more than 3 times in stool and frequent bowel movement more than 1.5 times after intake of αCD of 3g per day for 3 weeks.(Wacker Chemical Corporation)
⇒ αCD is assimilated slowly, and finally become nutrient of probiotics coexistence with various organic compounds and bacteria in intestinal environment.

αCD as prebiotics can be used to ingredient of synbiotics.