No. 29 Enhancement Effect on DPPH Radical Scavenging Activity of Astaxanthin-γ-CD Complex by the Addition of Vitamin E

Summary

Astaxanthin (AX) is a powerful antioxidant useful as an anti-aging agent. However, it is unstable after isolation in the presence of oxygen. We, therefore, previously reported the effective stabilization of AX by complexation with γ-CD in combination with vitamin E (VE).
In this study, the enhancing effects of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of AX and VE by the complexation of γ-CD were investigated. The activity of the mixture of both AX and VE complexes was found to be three fold higher than that of the non-complexed mixture.

Astaxanthin Oil Extract

H.Pluvialis induces encystment (carotogenesis), during which the cells produce high levels of astaxanthin as a protective mechanism against the environmental stress. The cells, with their high concentrations of astaxanthin, are then harvested.
As promoted proliferation of Astaxanthin, the cells are changed from green to red. Commercially as a natural source, astaxanthin is extracted from this encystment.

Antioxidant Activity of Astaxanthin

Solvent：DMF/CDCl3 (9 : 1)

Astaxanthin acts as antioxidant across cell membrane with the inclusion of hydrophilic and hydrophobic, so it's a more powerful antioxidant than other compounds.

Kazunaga Yazawa, et. al.,
SEIZANDO-SHOTEN PUBLISHING CO.,LTD.(2009)
ISBN:978-4-425-88481-0

Applications of Astaxanthin

Astaxanthin is recently the most remarkable carotenoid, and its applications aren't only fishing and animal industry but also human health foods and cosmetics.

DPPH Radical Scavenging Activity

The Actibity was Enhanced by Complexation with γ-CD !

[ ] : Compound Treated by Complexation

Synergy Effect by Complexation with γ-CD in the Activity

[ ] : Compound Treated by Complexation

The activity of the mixture of both astaxanthin and vitamin E complexes was found to be three fold higher than that of the non-complexed mixture.