Recent research finding
No.45 Antibacterial Activity of α-Cyclodextrin on Periodontal Pathogenic Bacteria


*This research was reported in 29th Cyclodextrin Symposium (Hoshi Univ., Tokyo, Sep. 6~7, 2012).

Dental Caries and Periodontal Disease

Recently, it is said that the oral health condition is deeply related with whole body diseases such as diabetes, and more attention has been paid on mouth care.

To suppress the increase of these bacteria is important, and many healing materials for oral care have been developed.

Functions of αCD

1. Anti-allergy
2. Anti-metabolic syndrome
3. Regulate the functions of the intestines
4. Antibacterial effect

Bacteriolysis Activity of CDs

It was found that αCD and methyl-βCD possess bacteriolysis activity against Bacillus strain.

methyl-βCD treated bacillus halodurans cell.
(Reference) Hui-Min Zhang, et al., Arch Microbiol ;190:605-609 (2008).

In this study, we explored about the antibacterial activity of αCD on P. gingivalis.


Minimum Inhibitory Concentration : MIC
MIC is the lowest concentration of a antibacterial reagent that will inhibit the visible growth of a bacterial.

Minimum Bactericidal Concentration : MBC
MBC is the lowest concentration of a antibacterial reagent required to kill 99.9% bacterial. It can be determined from broth dilution MIC tests comparison with subculture of agar media without the reagent.

Antibacterial Activity of αCD

  MIC (%)
MBC (%)
αCD 2.18 2.18
Incubation : 54 hours

αCD could inhibit the growth of P.gingivalis.

Antibacterial Activity of Various CDs

MIC and MBC of αCD on P.gingivalis : 2.18%

We compared activity of αCD to other CDs at 2.18% concentration. (incubation time : 54h, n=2)

Growth inhibition : αCD = methyl-βCD >> βCD


We investigated the antibacterial activity of various CDs at 2.18% concentration.
Growth inhibition was αCD = methyl-βCD >> βCD.

αCD could be applied not only as a dietary fiber, but also as a key ingredient of oral care products.