No. 45 Antibacterial Activity of α-Cyclodextrin on Periodontal Pathogenic Bacteria
This research was reported in 29th Cyclodextrin Symposium (Hoshi Univ., Tokyo, Sep. 6~7, 2012).
Dental Caries and Periodontal Disease
Recently, it is said that the oral health condition is deeply related with whole body diseases such as diabetes, and more attention has been paid on mouth care.
To suppress the increase of these bacteria is important, and many healing materials for oral care have been developed.
Functions of α-CD
- Anti-allergy
- Anti-metabolic syndrome
- Regulate the functions of the intestines
- Antibacterial effect
Bacteriolysis Activity of CDs
It was found that α-CD and methyl-β-CD possess bacteriolysis activity against Bacillus strain.
(Reference) Hui-Min Zhang, et al., Arch Microbiol ;190:605-609 (2008).
In this study, we explored about the antibacterial activity of α-CD on P. gingivalis.
Methods
- Minimum Inhibitory Concentration : MIC
MIC is the lowest concentration of a antibacterial reagent that will inhibit the visible growth of a bacterial. - Minimum Bactericidal Concentration : MBC
MBC is the lowest concentration of a antibacterial reagent required to kill 99.9% bacterial. It can be determined from broth dilution MIC tests comparison with subculture of agar media without the reagent.
Antibacterial Activity of α-CD
α-CD could inhibit the growth of P.gingivalis.
Antibacterial Activity of Various CDs
MIC and MBC of α-CD on P.gingivalis : 2.18%
→ We compared activity of α-CD to other CDs at 2.18% concentration. (incubation time : 54h, n=2)
Growth inhibition : α-CD = methyl-β-CD >> β-CD
Conclusion
We investigated the antibacterial activity of various CDs at 2.18% concentration.
Growth inhibition was α-CD = methyl-β-CD >> β-CD.
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α-CD could be applied not only as a dietary fiber, but also as a key ingredient of oral care products.