Recent research finding
No.53 Influence of Tocotrienol-γCyclodextrin complex in Various Stress Tolerance of Caenorhabditis elegans

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*This research was reported in 29th Cyclodextrin Symposium (Hoshi Univ., Tokyo, Sep. 6~7, 2012).

Background

Our company have an attract attention technology in functional food and personal care fields which raises the water solubility, stability and bioavailability of a functional ingredient by using the inclusion property of cyclodextrin (CD).

Recently, we focused "Tocotrienol" as a notable anti-aging ingredient and succeeded in the enhancement of its thermal stability and bioavailability by inclusion with γCD (* please refer to the newest result of research "38th" of our homepage for details.). We believe those results showed that "Tocotrienol-γCD complex" is useful as functional health food and cosmetics materials.

In this research, the prolongevity effects of Tocotrienol-γCD complex supplementation in Caenorhabditis elegans stimulated with various stress were explored.

Structures of tocotrienols

Tocotrienol

isomer R1 R2 R3
α CH3 CH3 CH3
β CH3 H CH3
γ H CH3 CH3
δ H H CH3

Appeal points of tocotrienol

Anti-metabolic activity Skin-whitening
Anti-oxidation Anti-wrinkle
Anti-cancer Hair growth

be more healthy
be more beauty
become more active

Fig 1 Effect of supplementation of tocotrienol-γCD on the survival of C. elegans stimulated with UV.

- Stress tolerance of C. elegans was enhanced by supplementation of tocotrienol-γCD complex under UV stress.

Fig 2 Effect of supplementation of tocotrienol-γCD on the survival of C. elegans stimulated with hydrogen peroxide.

- Stress tolerance of C. elegans was enhanced by supplementation of tocotrienol-γCD complex under hydrogen peroxide stress.

The prolongevity effects among tocotrienol isomers in C. elegans were compared.

Fig 3 prolongevity effects of tocotrienol isomers.

- All isomers of tocotrienol-γCD showed prolongevity effects in C. elegans.
- δ-tocotrienol-γCD was the most effective in all isomers.

Conclusion

Stress tolerances of C. elegans were enhanced by supplementation of tocotrienol-γCD complex under UV or hydrogen peroxide stress.
Each isoform of α-, γ-, or δ-tocotrienol-γCD complex was individually administered to C. elegans as a dietary supplement. The lifespan of worms supplemented with δ-tocotrienol-γCD was the most prolonged compared to that of worms supplemented with other isoforms.