No. 52 Extension of the solubilization method of hydrophobic ingredients-γ-cyclodextrin inclusion complexes
This research was reported in 29th Cyclodextrin Symposium (Hoshi Univ., Tokyo, Sep. 6~7, 2012).
Background
Our company has an attract attention technology in functional food and personal care fields which raises the water solubility, stability and bioavailability of functional ingredients by using the inclusion property of cyclodextrin (CD).
Recently, we constructed a method that could prepare aqueous solutions of hydrophobic ingredients such as CoQ10 and curcumin from its CD inclusion complexes by combination with some surfactants. These results have been widely applied in functional health foods and cosmetics (* please refer to the newest result of research "25th" and the "32th" of our homepage for details.).
In this report, we explored some combinations of surfactants and effectors for further extension of our solubilization methods.
Effective solubilization of hydrophobic material from its inclusion complex with bile acid.
Previously, we usually explored the solubilization effects of γ-CD inclusion complexes with sodium taurocholate or dipotassium glycyrrhizate as surfactants.
These surfactants have two characteristics such as
- They can act as an effector for the exclusion of hydrophobic active ingredients.
- They can form micelles to solubilize hydrophobic active ingredients.
In this study, we explored effective solubilization method of hydrophobic active ingredients by the combination of CD inclusion complex with effectors and surfactants for micelle formation for the extension of our original solubilization method.
Reagents
Effectors that can interact with γ-CD.
Micelle for solubilization of hydrophobic ingredients
Polyglyceryl fatty acid ester (DGOE) < Commonly-used emulsifier >
Hydrophobic ingredients
Results
Effective solubilization of CoQ10 was observed in the case of only its γ-CD complex with the combination of effector and surfactant for micelle formation.
Curcumin was also solubilized effectively from its γ-CD complex with the combination of effector and surfactant for micelle formation.
Conclusion
We achieved the effective solubilization of hydrophobic ingredients with not only previous one component system like sodium taurocholate but also present two components system consisting of effector and surfactants for micelle formation.
→ Our solubilization technology has high generality.
This technology can be useful for the developments of health supplements and drinks with high bioavailability.