No. 56 Development of δTocotrienol-γ-Cyclodextrin complex | CycloChem Bio Co., Ltd.
CycloChem Bio Co., Ltd.
Research findings
Research findings

No. 56 Development of δTocotrienol-γ-Cyclodextrin complex

This research was reported in 30th Cyclodextrin Symposium (Kumamoto, Sep. 12~13, 2013).


Our company have attractive technologies in functional food and personal care fields which raises the water solubility, stability and bioavailability of a functional ingredient by using the inclusion property of cyclodextrin (CD).

We focused "Tocotrienol" as a notable anti-aging ingredient and succeeded in the enhancement of its thermal stability and bioavailability by inclusion with γ-CD (* please refer to the newest result of research "38th" and "53th" of our homepage for details.).

Recently, a new Tocotrienol-γ-CD complex (δT3-γ-CD) containing very high amount of "δ-tocotrienol" as the most powerful antioxidant among tocotrienols has been developed. So in this research, stability and water solubility of δT3-γ-CD were evaluated for use as functional health food and cosmetics materials.

Structures of tocopherols and tocotrienols

Annatto seed oil

Extracted oil from annatto seed, it has the highest concentration of δ-tocotrienol, and is naturally tocopherol-free (Tocopherol interferes with tocotrienol benefits.).
Recently, we developed δ-tocotrienol-γ-CD complex powder "δT3-γ-CD" by using of annatto seed oil.

Appeal points of tocotrienol

→ Many health benefits of δT3 and γT3 which have fewer methyl group and show high anti-oxidant effect have been reported.

γ-CD assisting effective absorption of hydrophobic ingredients

Recently, we found the inclusion of hydrophobic ingredients such as CoQ10 and curcumin with γ-CD could support their absorption by incorporating easily into bile acid micelle in intestine.

Solubilization of δT3-γ-CD in the presence of bile acid was explored for enhancement of bioavailability of δT3.

δT3-γ-CD complex

Stability of δT3-γ-CD

@40℃, 75%RH

δT3-γ-CD has enough stability.

Solubility of δT3-CD with bile acid

The highest solubility of δT3 with bile acid was observed in the case of γ-CD complex.


  • δT3-CD prepared with Annatto seed oil and γ-CD was fine and high-dispersible powder.
  • δT3-γ-CD showed high stability @40℃, 75%RH for 6 months.
  • The highest solubility of δT3 with bile acid was observed in the case of γ-CD complex.

→ δT3-γ-CD can be useful for the developments of health or beauty supplements and drinks with high bioavailability.