Research findings

No. 48 Effect of Astaxanthin with Cyclodextrin Complex on Swiming Endurance of Mice

This research was reported in 29th Cyclodextrin Symposium （Hoshi Univ., Tokyo, Sep. 6~7, 2012).

Background

Our company have an attract attention technology in functional food and personal care fields which raises the water solubility, stability and bioavailability of a functional ingredient by using the inclusion property of cyclodextrin (CD).

Recently, we focused "Astaxanthin" as a notable anti-aging ingredient and succeeded in the dramatic enhancement of its stability, antioxidant capacity and bioavailability by inclusion with γ-CD (* please refer to the newest result of research "8th" and the "23th" of our homepage for details.). We believe those results showed that "Astaxanthin- γ-CD complex" is useful as functional health food and cosmetics materials.

In this research, the swimming endurance of mice was examined by supplementation of Astaxanthin-CD complex in comparison with non complexed Astaxanchin in order to see the effect of the enhanced bioavailability.

Structure of Axtaxanthin

Astaxanthin

CAS : [472-61-7] 3,3'-dihydroxy-β,β-carotene-4,4'-dioneC40H52O4
FW : 596.82

Effect of Complexation by γ-CD

Raw material for CD complex

Astaxanthin
ASTOTS-10O (Takeda Shiki：Ax 10% in Haematococcus pluvialis extract)

Test materials (Ax content%)
Control...ASTOTS-10O-β-CD complex (2%)
Ax-γ-CDcpx...ASTOTS-10O-γ-CD complex (2%)

Method
Eight-week-old mail rats were used for this test. The rats were administered test samples which contained Ax as 20mg/kgBW orally by use of stomach tube. Then, blood samples were collected from carotid arteries with time and their Ax contents were measured.

Results

JP2008-291150 (CycloChem Co., Ltd. Takeda Shiki co., Ltd. FUJIFILM Corporation

Swimming test of Mice

Materials

ASTOTS-10O* (Takeda Shiki co., Ltd.)
CAVAMAX W6 Food (α-CD：Wacker Chemical Corporation)
CAVAMAX W7 Food (β-CD：Wacker Chemical Corporation)
CAVAMAX W8 Food (γ-CD：Wacker Chemical Corporation)

Extract of Haematococcus pluvialis (10% Astaxanthin as free form) Astaxanthin exists as diester (15%), monoester (80%) and free form (5%). Rest part is consisted by fatty acid triglycerides (palmitic acid 16%, oleic acid 17%, linoleic acid 32%, alinolenic acid 16%, others).

Making test samples

ASTOTS-10O and CDs were mixed in DI water by homogenizer.
The mixture was dried by freeze drier, then test materials were obtained as powder.

Test method

To avoid circadian variations in physical activity, the experiments were 11:00 to 17:00. Five-week-old Std DDY mice were used for this test. To accustom the mice to swimming, all mice were given 2 weeks of preliminary period. Then, mice were divided into 10 groups (n=10) with equal mean of swimming time before experiments. The test samples were suspended in DI water. The mice were administered 0.5 mL of suspended solution orally by stomach tube 30min before swimming, The administration was once a day, five days a week, for five weeks.

Swimming conditions

Flow rate 7L/min, temperature 32℃ Maximum swimming time was defined as the failure to rise to the water surface to breathe within a 5 seconds period.

Dose

Control : Olive oil
Astaxanthin : 55mg/kgBW

An Adjustable-current swimming pool (ANITEC CO., Ltd.)

Water bath made by acrylic plastic (900x450x450mm)
It is designed for the evaluation of endurance capacity of mice. Water current on the surface is regulated and 5 mice can swim under the equal conditions.

Fig. 1. Maximum swimming time

→ Administration of Astaxanthin-CD complexes showed prolonged maximum swimming time.

Conclusion

Administration of all type of CD complexes of Haematococcus pluvialis extract which contains Astaxanthin showed prolonged swimming time compared with the control and non-treated Astaxanthin with CDs.

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CDs work on the bioavailability enhancement of aliphatic compound, such as Astaxanthin.