Recent research finding
No.48 Effect of Astaxanthin with Cyclodextrin Complex on Swiming Endurance of Mice

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* This research was reported in 29th Cyclodextrin Symposium (Hoshi Univ., Tokyo, Sep. 6~7, 2012).

Background

Our company have an attract attention technology in functional food and personal care fields which raises the water solubility, stability and bioavailability of a functional ingredient by using the inclusion property of cyclodextrin (CD).

Recently, we focused "Astaxanthin" as a notable anti-aging ingredient and succeeded in the dramatic enhancement of its stability, antioxidant capacity and bioavailability by inclusion with γCD (* please refer to the newest result of research "8th" and the "23th" of our homepage for details.). We believe those results showed that "Astaxanthin- γCD complex" is useful as functional health food and cosmetics materials.

In this research, the swimming endurance of mice was examined by supplementation of Astaxanthin-CD complex in comparison with non complexed Astaxanchin in order to see the effect of the enhanced bioavailability.

Structure of Axtaxanthin

Astaxanthin
CAS: [472-61-7] 3,3'-dihydroxy-β,β-carotene-4,4'-dioneC40H52O4
FW: 596.82

Effect of Complexation by γCD

Raw material for CD complex
Astaxanthin:
ASTOTS-10O (Takeda Shiki:Ax 10% in Haematococcus pluvialis extract)

Test materials (Ax content%):
Control...ASTOTS-10O-βCD complex (2%)
Ax-γCDcpx...ASTOTS-10O-γCD complex (2%)

Method: Eight-week-old mail rats were used for this test. The rats were administered test samples which contained Ax as 20mg/kgBW orally by use of stomach tube. Then, blood samples were collected from carotid arteries with time and their Ax contents were measured.

Results:

JP2008-291150 (CycloChem Co., Ltd. Takeda Shiki co., Ltd. FUJIFILM Corporation

Swimming test of Mice

Materials:
ASTOTS-10O* (Takeda Shiki co., Ltd.)
CAVAMAX W6 Food (αCD:Wacker Chemical Corporation)
CAVAMAX W7 Food (βCD:Wacker Chemical Corporation)
CAVAMAX W8 Food (γCD:Wacker Chemical Corporation)

*Extract of Haematococcus pluvialis (10% Astaxanthin as free form) Astaxanthin exists as diester (15%), monoester (80%) and free form (5%). Rest part is consisted by fatty acid triglycerides (palmitic acid 16%, oleic acid 17%, linoleic acid 32%, alinolenic acid 16%, others).

Making test samples:
ASTOTS-10O and CDs were mixed in DI water by homogenizer.
The mixture was dried by freeze drier, then test materials were obtained as powder.

Test method:
To avoid circadian variations in physical activity, the experiments were 11:00 to 17:00. Five-week-old Std DDY mice were used for this test. To accustom the mice to swimming, all mice were given 2 weeks of preliminary period. Then, mice were divided into 10 groups (n=10) with equal mean of swimming time before experiments. The test samples were suspended in DI water. The mice were administered 0.5 mL of suspended solution orally by stomach tube 30min before swimming, The administration was once a day, five days a week, for five weeks.

Swimming conditions: Flow rate 7L/min, temperature 32℃ Maximum swimming time was defined as the failure to rise to the water surface to breathe within a 5 seconds period.

Dose:

Control: Olive oil
Astaxanthin: 55mg/kgBW

An Adjustable-current swimming pool (ANITEC CO., Ltd.):
Water bath made by acrylic plastic (900x450x450mm)
It is designed for the evaluation of endurance capacity of mice. Water current on the surface is regulated and 5 mice can swim under the equal conditions.

Fig 1

Maximum swimming time

Significanto difference against control: * p<0.05

Test materials:
①...Olive oil
②...ASTOTS-10O
③...ASTOTS-αCD complex
④...ASTOTS-βCD complex
⑤...ASTOTS-γCD complex

Administration of Astaxanthin-CD complexes showed prolonged maximum swimming time.

Conclusion

Administration of all type of CD complexes of Haematococcus pluvialis extract which contains Astaxanthin showed prolonged swimming time compared with the control and non-treated Astaxanthin with CDs.

CDs work on the bioavailability enhancement of aliphatic compound, such as Astaxanthin.