No. 70 Effect of the combination of α-cyclodextrin and arabinose on blood glucose level after oral administration of sucrose
This research was done through collaboration with Kobe University, Kyoto Pharmaceutical University and Kobe woman's University, and reported in 31th Cyclodextrin Symposium (Shimane, Sep. 11~12, 2014).
Background
Our company has an attract attention technology in functional food and personal care fields which raises the water solubility, stability and bioavailability of functional ingredients by using the inclusion property of cyclodextrin (CD).
Diabetes mellitus is a serious health problem and diabetic patients increase dramatically all over the world. AGEs (advanced glycation end products) produced by hyperglycosemia are one of the factors to cause a complication of diabetes. Therefore it is important to inhibit the rapid elevation of postprandial blood glucose level.
Because of its high water solubility and non-digestible property, α-CD can be taken as a water-soluble dietary fiber. Indeed, daily feed intake of α-CD shows some anti-metabolic syndrome effects, improvements of the intestinal environment and anti-allergy effect. We also reported some interesting results supporting those effects (* please refer to the newest result of research "16th", "42th", "44th" and "50th" of our homepage for details.).
Functions of α-CD (human trial)
- Anti-metabolic syndrome
Lowering effect of fat and cholesterol
Inhibition effect against an elevation in blood glucose level - Regulation of intestinal functions
- Anti-allergy
Atopic dermatitis, Cedar pollen allergy, Asthma
Purpose
α-CD was previously reported as a inhibitor on elevation of blood glucose level after administration of sucrose to mice in our homepage "the newest result of research 42th".
We suggested that α-CD inhibited sucrase with competitive mechanism.
Arabinose is also known as a sucrase inhibitor with non-competitive mechanism.
So, in this study, the inhibition effect on elevation of blood glucose level after oral administration of sucrose by the combination of α-CD and arabinose was explored.
Method
- The mice were fasted for 14 hours and administered each sample orally.
- Blood sample was taken from the tail followed by measuring blood glucose level (0min, 15min, 30min, 60min, 90min and 120min).
Result
Fig. 1
A : Changes of blood glucose level (BGL) after oral administration of sucrose
B : Area under the two hour blood glucose level curve (AUC).
Significant inhibition effect on elevation of BGL was observed in Ara group.
Enhanced inhibition effect on elevation of BGL was observed in Ara+CD group.
Conclusion
The combination of arabinose and α-CD can be useful as an enhanced dietary fiber for the inhibition of blood glucose level after oral administration of sucrose.